Fig. 3

Self-renewal of placental CD117⁺ TSCs in vitro. CD117⁺ TSCs were plated in a 100-mm dish at limited dilution of one cell every 60 mm² for 14 days. a Representative image of multicellular clones stained for crystal violet (left, purple), higher magnification of a clone staining for crystal violet (middle, purple), and phase-contrast image of an entire clone (right). b Representative image of a clone staining positive for CD117 (green), DAPI for nuclear staining. c After expansion, 97% of clonal cells are positive for CD117 as represented in flow cytometry histogram, gray for Alex 488 isotope control, red for CD117 Alex 488 staining. d Representative image of clonal cells staining positive for caudal type homeobox 2 (CDX2; green, left), DAPI for nuclear staining (blue, middle), and merged image (right)