Fig. 8

Differentiation of CD117⁺ TSCs into three germ layers in vivo. Under ultrasound guidance (left column), CD117⁺ TSCs pre-labeled with PKH67 (green) were injected in utero into lung, myocardium, or the sub-retinal space of E13.5 mouse fetuses. Lungs, hearts, and eyes were harvested at day 1 after nature birth for histological assessment. Representative merged images of exogenous cells (TSCs, green), DAPI (blue), and immunofluorescence staining for a marker of lung alveolar epithelium, SPC (red, upper row), or a marker of cardiomyocytes, cTnI (red, middle row), or a marker of retinal photoreceptors, Rho (red, lower row). The third and fourth columns show respectively higher power magnification images of the area in the white boxes of the second column