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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Insulin-like growth factor-II overexpression accelerates parthenogenetic stem cell differentiation into cardiomyocytes and improves cardiac function after acute myocardial infarction in mice

Fig. 1

Parthenogenetic stem cell (PCS) generation coexpressing insulin-like growth factor-II (IGF-II) and enhanced green fluorescent protein (eGFP)-tagged myosin heavy chain (MHC). a Schematic diagram of parthenogenetic stem cell (PCS) transfection, selection, differentiation, cardiomyocyte enrichment, and engraftment. Undifferentiated embryonic stem cell (ESC) line D3 and mouse-derived ESCs were used as controls and underwent the same procedures. b Mouse-derived PCSs were sequentially and stably transfected with MHC-neor/pGK-hygror and MHC-eGFP via electroporation at 180 V. Eight PSC clones (#1–8) were generated. c Semi-quantitative PCR was conducted to determine MHC-neor and MHC-eGFP expression in the genome. d Clones #2 and #6 containing MHC-neor and MHC-eGFP were stably transfected with pME 18 s-IGF-II via electroporation. Semi-quantitative PCR determined IGF-II and Nanog expression in the genome. e MHC-neor(/pGK-hygror) and MHC-eGFP expression were determined in multiple passages of clone #6 derivatives

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Stem Cell Research & Therapy

ISSN: 1757-6512