Table 2 The primer sequences for RT-qPCR
Gene | Sequence (5′-3′) |
|---|---|
miR-495 | Forward: GCGCGTGAGCAGGCTGGAGAAATT Reverse: AAACAAACATGGTGCA |
HOXC6 | Forward: ACAGACCTCAATCGCTCAGGA Reverse: AGGGGTAAATCTGGATACTGGC |
TGF-β | Forward: CAACGCCATCTATGAGAAAACC Reverse: AAGCCCTGTATTCCGTCTCC |
TGFβRI | Forward: ATTACCTGGACATCGGCA AC Reverse: TTGGGCACCACATCATAGAA |
Smad4 | Forward: GTGGCTGGTCGGAAAGGATT Reverse: ACTGGCAGGCTGACTTGTGG |
TGFβRII | Forward: ACTTGACCTGTTGCCTGTGTGAC Reverse: CTGGCTTCAACGCCTTTCACCTCA |
N-cadherin | Forward: TCATTGCCATCCTGCTCTGCAT Reverse: AGTTGTTTGGCCTGGCGTTCTT |
Vimentin | Forward: AAAGTGTGGCTGCCAAGAACCT Reverse: ATTTCACGCATCTGGCGTTCCA |
E-cadherin | Forward: TTAGGTTAGAGGGTTATCGCGT Reverse: TAACTAAAAATTCACCTACCGACC |
U6 | Forward: CGCTTCACGAATTTGCGTGTCAT Reverse: GCTTCGGCAGCACATATACTAAAAT |
GAPDH | Forward: ACAGTCAGCCGCATCTTCTT Reverse: GACAAGCTTCCCGTTCTCAG |