Fig. 3

miR-765 inhibits hMSC osteogenic differentiation. Alizarin Red S staining was employed as a means of assessing matrix mineralization. Scale bar, 50 μm. a miR-765 overexpression was associated with decreased matrix mineralization. b Cell in which miR-765 had been knocked down exhibited more intense staining consistent with increased mineralization. c, d Differences in mineralization staining intensity were significant among these groups of cells. Data are expressed as means ± SD (X ± SD, n = 3). **P < 0.01 vs. negative controls, respectively