Fig. 5

miR-144 impedes NSCLC cell proliferation and colony formation and reduces the number of S phase-arrested cells by downregulating CCNE1 and CCNE2. a The expression of proliferation-related factors detected by western blot analysis. b Cell proliferation assessed by EdU assay (× 200). c Cell cycle progression identified by flow cytometry. d Number of colony formation assessed by colony formation assay. *p < 0.05 vs. A549 cells co-transfected with NC inhibitor + sh-NC. ^#p < 0.05 vs. A549 cells co-transfected with NC inhibitor + sh-CCNE1 + sh-CCNE2 group. The above measurement data were expressed as mean ± standard deviation. Data among multiple groups were analyzed by one-way ANOVA, followed by a Tukey post hoc test. The experiment was repeated in triplicate