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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Targeted silencing of miRNA-132-3p expression rescues disuse osteopenia by promoting mesenchymal stem cell osteogenic differentiation and osteogenesis in mice

Fig. 3

Effects of gravitational mechanical unloading on osteogenic differentiation of BMSCs. BMSCs were first exposed to a clinostat-based gravitational mechanical unloading environment for 0 h, 24 h, 48 h, 72 h and was then cultured with osteogenic medium for 4 days. a Gene expression of Runx2, Osx, and Alp. b Protein activity of ALP. c Protein expressions of RUNX2 and OSX. d The expression level of miRNA-132-3p in BMSCs after exposing to gravitational mechanical unloading (non-osteo, without osteogenic induction; osteo, further induced by an osteogenic medium). Values are shown as mean ± SD, n = 3. *P < 0.05, **P < 0.01. NS, no significant

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Stem Cell Research & Therapy

ISSN: 1757-6512