Fig. 1

Characterization of NMCs. a Myogenic differentiation ability of NMCs and myoblasts was assessed by culturing cells under myogenic differentiation conditions and immunohistochemistry (scale bar 50 μm). b–d Expression of cell surface and intracellular markers in NMCs, myoblasts, fibroblasts, and MSCs was analyzed by flow cytometry. Isotype control is shown in blue, and the antibody-stained sample is shown in red. e Double staining of CD56 and cell surface markers highly expressed in NMCs. The histogram shows the positive ratio of each marker in the CD56-negative population. NMCs, non-myogenic cells; MSC, mesenchymal stem cell; MHC, Myosin heavy chain