Fig. 2

hPO characterization. a Growth curve of hPOs; data are means ± SEM (n = 4/time point; right panel). b Representative bright-field images of hPOs from day 0 to day 7. Scale bar, 500 μm. c, d Mean (±SEM) diameter and area in a single passage (days 1, 2, 4, and 7). At least 100 organoids were measured. e, f Mean (±SEM) glucose consumption and lactate production at study time points in a single passage (n = 3). g Immunophenotypic analysis of adult islet-depleted pancreas and hPOs at different passages to evaluate ductal, acinar, mesenchymal, hematopoietic and endothelial markers. Data are expressed as mean ± SEM (n  =  3/group). Two-way analyses of variance (ANOVAs) followed by Newman-Keuls post hoc tests for multiple comparisons was used. **p < 0.01; ***p < 0.0001. h Representative flow cytometry density plots of physical parameters and acinar and ductal cell morphologies at P5. i Mean gene (±SEM) expression of ductal, acinar, mesenchymal, hematopoietic, endothelial, and pancreatic progenitor markers in hPOs (n = 4)