Fig. 2

Structural recapitulation of human lacrimal gland (LG) organoids. A Immunofluorescence staining for specific Markers, such as vimentin (VIM), E-cadherin (E-CAD), aquaporin5 (AQP5), alpha-smooth muscle actin (α-SMA), and lysozyme (LYZ), was performed in human LG tissue and organoid cultured in LG organoid medium (LGOM). B In the organoid, the proliferating cells were confirmed via BrdU assay. At 4 days after BrdU treatment, proliferating cell (BrdU and Ki76) and LG-specific (AQP5 and VIM) markers) were detected in organoids (Magnification; 100×, Scale bar; 50 µm). C TEM analysis was performed to confirm cellular ultrastructure in the organoid. Acinar cells (up; white dotted line) and secretory granules (down; red arrowhead) were detected in the LG organoid (Scale bar; 5 µm)