Fig. 1

Medium-throughput screening identifies several unique compound classes that increase retinal stem and progenitor cell number. a Schematic overview of the MTS pipeline to detect RSPC expanding compounds. b–e Quantification as a percent of control (POC) of all compounds that met the hit criteria of having a relative number of nuclei or total area of actin-GFP expression over 3xSD above the 1× control mean. Hits that were identified in multiple criteria and/or screens are color-coded. b, c Seven compounds in screen 1 were found to be hits in both the number of nuclei and actin-GFP area and were selected as lead compounds. 1× control: N = 80 technical replicates. 2× control: N = 12 technical replicates. Compounds: N = 1 technical replicate. d, e Eight compounds in screen 2 were found to be hits in both the number of nuclei and actin-GFP area and were selected as lead compounds. 1× control: N = 80 technical replicates. 2× control: N = 15 technical replicates. Compounds: N = 1 technical replicate. f A dose-response assay of the hit compounds identified via MTS. N = 3 for all compounds at each concentration. Data are mean ± SEM