Fig. 2

DNA damage and apoptosis associated with the inhibition of DKC1 in human hematopoietic stem and progenitor cells. Cord blood CD34⁺ cells were transduced with specific anti-shRNA-LVs and maintained in liquid cultures for 10 days (see details in “Materials and methods” section). a Analysis of DNA damage in CD34⁺ cells transduced with specific shRNA LVs. Cells with more than 10 γH2AX foci per cell are shown (n = 3). b Representative Western blot (WB) assays for phosphorylated p53 (upper WB) and fragmented caspase 3 (lower WB) expression using β-actin as control. Quantification appears in italics below the images as the ratio of expression in relation with the control protein. c Increased expression of p21 after transduction of CD34⁺ cells with specific shRNA-LVs (n = 6). Raw data are shown in Suppl. Table 2. d Fold increase of apoptotic cells (Annexin V⁺) in comparison with the scrambled control condition (n = 3). Data are expressed as mean ± SEM. Asterisks indicate significant differences determined by Student’s t test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)