Fig. 7

Immunohistochemical analysis of newly formed bone tissue. a Cells with positively stained cytoplasm were observed as brown nodes, while the cell nuclei were observed as blue nodes. The expression levels of TIMP2 and MMP2 in Lenti-miR-672, Lenti-as-miR-672, and Lenti-miR-NC groups. b Positive cell ratios of TIMP2 and MMP2 in newly formed bone were determined by dividing the number of TIMP2- and MMP2-positive cell by the total cell number. Scale bar 200 μm. Statistical significances were determined by unpaired Student’s t test using GraphPad Prism Version 8. *P < 0.05, **P < 0.01