Fig. 1

Dox induced cell apoptosis in hUC-MSCs. a The workflow of doxorubicin cytotoxicity model in vitro. b Representative immunofluorescence images of hUC-MSCs treated with doxorubicin for 4, 6, 8, 10, and 12 h. Normal hUC-MSCs were taken as control group. Intracellular accumulation of doxorubicin, revealed by the red fluorescence, was increasing with treatment period being prolonged. Scale bars, 20 μm. c Quantification of doxorubicin in hUC-MSCs of incremental treatment period, which showed consistent trend with immunofluorescence image. n = 3, ^*P < 0.05 versus Blank groups. d Representative immunofluorescence images of stained cleaved-caspase 3 (green) in hUC-MSCs with doxorubicin treating for 6 h. Cell nucleus were also stained with DAPI (blue) for colocalization. Scale bars, 20 μm. e The cell survival rate of hUC-MSCs analyzed by CCK-8 was decreasing in a time-dependent manner. n = 3, ^*P < 0.05 versus Blank groups