Fig. 2

The OPC apoptosis was induced after they were incubated for 24 h with the conditioned medium (CM) from OGD-ECs. a Representative images of OPCs with TUNEL staining, scale bar = 200 μm. b OPC apoptosis induced by the CM from OGD-ECs was detected by FACS. c Quantitative analysis of apoptotic cell number and apoptosis rate of OPCs, respectively. Comparing the mean ± SD (n = 4, triplicates per group), *P < 0.05, ***P < 0.001 vs. DMEM/F12 group; ^#P < 0.05 vs. OGD-EC-CM group. Data were analyzed using Student’s t test