Fig. 3

MSCs-Exo-derived miR-223 inhibited ICAM-1 expression. Bioinformatic analysis indicated that miR-223 regulated the expression 3′-UTR of ICAM-1 (a). Transfection of miR-223 mimic induced significant downregulation of ICAM-1 (b). High expression of miR-223 by qPCR after transfection of 100 nM miR-223 mimics (c). ICAM-1 mRNA and protein expression showed decrease as revealed by qPCR and Western blot (d, e). After 24 h co-culturing of MSCs-Exo (2 μg) with HUVECs, the expression of ICAM-1 in HUVECs showed decrease as revealed by qPCR (f) and Western blot (g). Data were presented as mean ± SEM. Measured in three independent experiments. *P < 0.05, ***P < 0.001