Fig. 4

MiR-223 impaired T cell crawling, adhesion, and transmigration in vitro. Measurement of CD4⁺ Th1 differentiation using flow cytometry (a). Adhesion assay for the Th1 cells stained with green CellTracker™ and co-cultured with mLECs transfected miR-223 mimics (100 nM) or NC for 45 min (b). The average number of green adherent cells in transfected miR-223 group showed significant decrease (c). Crawling assay for the Th1 cells stained with green CellTracker™ seeded on the mLEC transfected miR-223 mimics or NC for 20 min. Time-lapse imaging was performed on Operetta CLS™ (d). Compare with NC group, there was significant decrease in the crawling speed and distance of T cells in miR-223 mimics group (e, f). The transmigration assay results indicated the number of Th1 cells stained with green CellTracker™ in miR-223 group was significantly lower compared with that of normal control (g). Data are presented as mean ± SEM. Measured in two independent experiments. **P < 0.01, ***P < 0.001