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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Exosomes derived from miR-301a-3p-overexpressing adipose-derived mesenchymal stem cells reverse hypoxia-induced erectile dysfunction in rat models

Fig. 1

a ADSCs were collected from adipose tissues of SD rats. ADSCs displayed a typical cobblestone-like morphology under a microscope. b Adipose cells were confirmed with Oil Red O staining. c ADSCs were positive for the mesenchymal stem cell (MSC) markers CD29, CD34, CD44, CD90, and CD105. d Flow cytometry analysis of the surface markers in ADSCs. e Sequence of rno-miR-301a-3p and has-miR-301a-3p. f The expression levels of has-miR-301a-3p in serum samples from patients (n = 30, ***p < 0.001). g The expression levels of rno-miR-301a-3p in serum samples from SD rats (n = 8, **p < 0.01). h Transmission electron microscopy analysis of exosomes secreted by ADSCs (scale bar = 100 nm). i The particle size of the exosomes secreted by ADSCs was measured by nanoparticle tracking analysis. j Protein levels of TSG101, CD9, and CD63 in ADSC and ADSC-derived exosomes as determined by Western blotting. k RT-qPCR results of miR-301a level in ADSC and ADSC-derived exosomes. Data are expressed as mean ± SD (n = 3 for ADSC analysis; ***p < 0.001)

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Stem Cell Research & Therapy

ISSN: 1757-6512