Fig. 1

Akt is activated by TGFβ2 and inhibited with MK-2206 as a function of time. a–g Quantified western blot band densitometry showing the ratio of P-Akt to Akt as a function of time. The ratio of P-Akt to Akt was significantly increased by TGFβ2 treatment at b 30 m, c 60 m, and d 24 h. Akt activation levels in MSCs were significantly decreased by MK-2206 at b 30 m, c 60 m, d 24 h, f 7 d, and g 14 d. h Representative western blot showing MK-2206 prevents Akt activation at 60 m, while TGFβ2 increases Akt phosphorylation. P-P70S6K and P70S6K remained unaffected. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Bars = mean ± standard deviation