Fig. 4
From: Akt signaling is activated by TGFβ2 and impacts tenogenic induction of mesenchymal stem cells
Smad3 inhibition has limited impact on Akt activation. Quantified western blot band densitometry showing the ratio of P-Akt to Akt in MSCs treated with SIS3 to block Smad3 signaling. The ratio of P-Akt to Akt was not significantly altered by SIS3 at a 15 m, b 30 m, and d 24 h. c Akt activation trended higher in cells treated with TGFβ2 and SIS3 + TGFβ2 at 60 m (p = 0.09). e Akt activation levels were significantly decreased in all conditions except vehicle controls by 3 d. *p < 0.05, **p < 0.01. Bars = mean ± standard deviation