Fig. 4

Givi-MPC decreased inflammation and muscle necrosis in Mdx/SCID mice 7 days after CTX injury. a Representative images of HE and Masson's trichrome staining in Mdx/SCID mice after human myoblasts or control MPC or Givi-MPC transplantation 7 days after CTX injury. Black arrows point at infiltrated inflammatory cells. b, c Quantification of TA muscle fiber necrosis after treatment with MPC 7 days after CTX injury. Human myoblasts: n = 3; control-MPC: n = 4; Givi-MPC: n = 5. d Quantification of CD68-positive cells in TA muscle following MPC transplantation 7 days after CTX injury. n = 4. e Representative images of macrophages (red, CD68) and human cells in TA CTX-injured muscle of Mdx/SCID mice following MPC transplantation. The boxed area is shown below at high magnification. Bar = 100 μm