Table 1 Primers for qRT-PCR and oligonucleotide
Name | Sequence | |
|---|---|---|
qPCR | ||
 miR-34a | 5′-UGGCAGUGUCUUAGCUGGUUGUU-3′ | |
 U6 | F | 5′-CCTGCTTCGGCAGCACA-3′ |
R | 5′-AACGCTTCACGAATTTGCGT-3′ | |
|  | F | 5′- CCCAGCACAATGAAGATCAAGATCAT-3′ |
R | 5′- ATCTGCTGGAAGGTGTACAGCGA-3′ | |
Oligonucleotide | ||
 miR-34a mimic | UGGCAGUGUCUUAGCUGGUUGUU CAACCAGCUAAGACACUGCCAUU | |
 Negative Control (NC) mimic | UUCUCCGAACGUGUCACGUTT ACGUGACACGUUCGGAGAATT | |
 miR-34a inhibitor | UGGCAGUGUCUUAGCUGGUUGUU | |
 NC inhibitor | CAGUACUUUUGUGUAGUACAA | |
 siRNA-SIRT1 | GCACCGAUCCUCGAACAAUTT AUUGUUCGAGGAUCGGUGCTT | |
 siRNA-NT | UUCUCCGAACGUGUCACGUTT ACGUGACACGUUCGGAGAATT | |