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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Hollow-fiber bioreactor production of extracellular vesicles from human bone marrow mesenchymal stromal cells yields nanovesicles that mirrors the immuno-modulatory antigenic signature of the producer cell

Fig. 7

Analysis of EVs produced in the hollow-fiber system from hBM-MSCs confirms an immune-modulatory cargo profile. a Milliplex cytokine analysis of EVs isolated from each hBM-MSC donors (N = 4 donors; hBM-MSC-81RB/48RB/55RB/85RB) over several days (days 2, 14, and 24) confirms packaging of anti-inflammatory and angiogenic factors. Protein lysates were generated from EV samples collected at days 2, 14, and 24 of the 25-day EV production period. Heat map showing the analysis of 29 cytokines and growth factors from the four donors at the three collection time points. b Quantitative analysis of the cytokine and growth factor detection of the mean of the four hBM-MSC donors (N = 4 donors; hBM-MSC-81RB/48RB/55RB/85RB) at the three time points (days 2, 14, and 24) is represented. Each dot represents N = 4 donors, each analyzed in duplicates. Statistical significance was analyzed by a two-way analysis of variance (ANOVA) with Tukey’s post hoc analysis. A p value of < 0.05 was considered statistically significant and significant differences are marked with a triple asterisk (p < 0.001)

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Stem Cell Research & Therapy

ISSN: 1757-6512