Fig. 5

Reduced neutrophil infiltration in inflamed pads in mice treated with Ad-MSCs expressing CXCR4 and/or IL10 compared to WT-MSCs. a Representative images of inflamed pads from LPS and Ad-MSC treated mice 48 h after infusion of WT or mRNA-transfected MSCs. b Representative histological images of inflamed pads 48 h after injection of WT or mRNA transfected MCSs. Five sections/pad were stained with hematoxylin and eosin (bar = 200 μm). c Representative flow cytometry histograms showing the proportion of murine CD45⁺ cells in inflamed pads from mice treated with WT and mRNA-transfected MSCs. d Quantification of the flow cytometry analysis of CD45⁺ cells shown in b. e Representative flow cytometry analysis of neutrophils (Ly6C⁻ Ly6G⁺ CD11b⁺ cells) in CD45⁺ cells infiltrating the inflamed pads from mice treated with WT and mRNA-transfected MSCs. f Quantitation of the flow cytometry analysis of neutrophil infiltration in the inflamed pads from mice treated with WT and mRNA-transfected MSCs. Bars represent the mean ± SEM of at least n = 3 mice/group, and statistical significance was determined using Tukey’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001