Fig. 2

MPC implantation model. a Bioluminescence intensity (BLI) over time of a representative mouse injected with fixed number and volume (1 × 10E6 cells in 25 μL+ 5 μL beads) of Luciferase-MPC and fluorescent beads into the tibialis anterior muscles either by a single-needle (left hindlimb) or 4-needle applicator (right hindlimb). BLI images representing 30 min measurements of luciferase specific photon emission set to a standardized scale (rainbow). b Luciferase signal quantification (area under the curve; AUC) of 4 mice receiving 1 × 10E6 Luciferase-MPC either by 1-needle injection (left M. tibialis anterior) or 4 needle injection (right M. tibialis anterior). Luciferase signals quantified by background corrected area under the curve of 30 min (1 measurement per minute) analysis on 7, 29, 56, and 71 days post cell implantation. Data presented as mean and SD. c Section of TdTomato MPC and fluorescent beads-injected mouse stained with anti-TdTomato and anti-Laminin antibodies as well as DAPI (Scale bar = 200 μm). d Luciferase signal quantification by cell dose of two mice either receiving 1 × 10E5 or 1 × 106E6 cells injected in the right and left m. gastrocnemius, respectively on days 1, 7, and 35 post implantations demonstrated as % AUC of low cell dose at day 1. AUC, area under the curve; BLI, bioluminescence; DAPI, 4′,6-diamidin-2-phenylindol; MPC, myogenic progenitor cell; SD, standard deviation