Fig. 2

DPSC telomere lengths during extended culture with or without exogenous H₂O₂ (50–200 μM) treatment. Representative images of TRF analysis (determined by Southern blotting) and mean telomere lengths (ImageJ® analysis), determined for a high proliferative sub-population, A1 (10–25PDs and 40–65PDs) and b–d low proliferative sub-populations A2 (2–10PDs), C3 (2–10PDs), and D4 (2–10PDs). Left- and right-hand lanes represent separated DIG-labeled telomere length standards (kb, in Kit). CTRL represent telomere length positive control (in Kit). N = 3, values in graphs represent the mean ± SEM. ***p < 0.001. N.S. = Non-significant