Fig. 3
Senescence-related marker detection during extended DPSC culture with or without exogenous H2O2 (50–200 μM) treatment. a, b Representative SA-β-galactosidase microscopy images and % positively stained cell calculations, for high proliferative sub-population, A1 (58–80PDs) and low proliferative sub-population, D4 (2–10PDs). Scale bar 100 μm, × 100 magnification. N = 3, values represent the mean ± SEM. ***p < 0.001 versus untreated DPSC controls. Characterization of senescence marker (p53, p16INK4a, p21waf1, hTERT) expression for c high proliferative sub-population, A1 (10–25PDs and 40–65PDs), d, e low proliferative DPSC sub-populations C3 (2–10PDs) and D4 (2–10PDs). β-actin was used as the housekeeping gene. Right-hand lanes represent separate total human RNA-positive controls, water and RT-negative controls. bp = base pairs