Fig. 5

Mesenchymal, embryonic and neural crest stem cell marker expression during extended DPSC culture with or without exogenous H₂O₂ (50–200 μM) treatment. a High proliferative sub-population, A1 (10–25PDs and 40–65PDs), and b–d low proliferative sub-populations A2 (2–10PDs), C3 (2–10PDs), and D4 (2–10PDs). β-actin was used as the housekeeping gene. Right-hand lanes represent separate total human RNA-positive controls, water, and RT-negative controls. bp = base pairs. N = 3