Regulation of connective tissue growth factor expression by miR-133b for the treatment of renal interstitial fibrosis in aged mice with unilateral ureteral obstruction

Table 1 Comparison of fluorescence intensity of α-SMA and E-cadherin in HK2 cells under different stimulation conditions

Group	Number of experiments	Average fluorescence intensity of cells
Group	Number of experiments	α-SMA	E-cadherin
Control	3	0.021 ± 0.003	0.175 ± 0.028
TGF-β1	3	0.103 ± 0.043*	0.013 ± 0.001*
TGF-β1+miR-133b	3	0.041 ± 0.005*^,#	0.067 ± 0.009*^,#
TGF-β1+NC-miR	3	0.092 ± 0.004*	0.009 ± 0.001*

The immunofluorescence experiment was performed in triplicate, and five fields of vision were selected for each group. The fluorescence intensity of cells was observed under a confocal microscope (× 100); *P < 0.05 versus the control group; ^#P < 0.05 versus the TGF-β1 group

ISSN: 1757-6512