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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: METTL3-mediated m6A modification regulates cell cycle progression of dental pulp stem cells

Fig. 1

Characterizations and regenerative capacity of DPSCs. a Mesenchymal stem cell antigen (CD29, CD44, CD90) and hematopoietic cell antigen (CD34, CD45) expressed in DPSCs were detected by flow cytometry. b Alkaline phosphatase (ALP) straining of DPSCs after 7 days of odontogenic induction. c mRNA expression of early odontogenic markers COL1, ALP, and RUNX2 analyzed by PCR. d Calcium mineralization formation observed by alizarin red S staining (ARS) at day 14 in DPSCs. e mRNA expressions of late odontogenic genes-BSP, OCN, DSPP were assayed by PCR. f Accumulation of lipid vacuoles was detected by oil O staining. g mRNA expression of CREB and LPL after adipogenic induction detected by PCR. Significance was determined via Student’s t test analysis of variance (n = 3); data are represented as mean ± SD. *p < 0.05. **p < 0.01. ***p < 0.001

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