Fig. 4
Osthole promotes the expression of OPG through activation of β-catenin signaling. a OPG immunostaining was performed in L5 sections of 12-month-old C57BL/6 mice. b Osthole increased the OPG protein level in aged mice. c β-catenin immunostaining was performed in L5 sections of 12-mont-hold C57BL/6 mice. d Osthole increased the β-catenin protein level in aged mice. e Osteoblasts were isolated from 3-day-old β-cateninfx/fx mice, infected with Ad-Cre or Ad-GFP, and treated with or without 100 mM osthole. After 2 days, the expression of OPG was detected using real-time PCR assay. *P<0.05. f Osteoblasts were isolated from 3-day-old OPG−/− mice and the littermates of OPG+/+ mice were treated with osthole (100 μM) or vehicle for 2 days; the expression of β-catenin was detected using the Western blot assay