Table 2 Oligonucleotide primers and PCR conditions for CHIP-PCR
Genes | Forward primer | Reverse primer | Annealing |
|---|---|---|---|
Homo COL2A1 | TGCAGGGAAGGGCTAAAAGA | GGAGCCCACAGAGATTCAGA | 60 |
Homo ACAN | CTCGAACTCAGTCCCACCTT | ACCTGCCCCTAACCAAAGAT | 60 |
Homo TGFβRI | ATCGGGAAGGGGTTTGAGAG | AGATCCTGAGCCCAAACACA | 60 |
Homo Smad2 | CGAGTGCCTAAGTGATAGT | AGACTGAGCCAGAAGAGC | 60 |
Homo Smad3 | GGGCTTTGAGGCTGTCTA | CCAACCCGATCCCTTTAC | 60 |
Rat TGFβRI | ACTGGAATTTGAGGAGGGCA | TAGACCCGCTCCTCAATTCC | 60 |