Fig. 4

ALPL regulates the odontoblastic differentiation of DPSCs. a Alizarin Red staining was performed in DPSCs from the health and HPP groups after cultured in odontoblastic medium for 3 weeks. b The expression of DMP1 and DSPP was examined on gene and protein level in DPSCs from the health and HPP groups after cultured in odontoblastic medium for 7 days (n = 3). To evaluate whether ALPL could regulate DPSC differentiation into odontoblasts, we upregulated or downregulated the expression of ALPL in HPP DPSCs with pLenti-ALPL or normal DPSCs with pLko.1-ALPL shRNA lentiviral transduction. c, e Alizarin Red staining was performed in DPSCs from health and health with pLko.1-ALPL shRNA (c), or DPSCs from HPP and HPP with pLenti-ALPL (e) after cultured in odontoblastic medium for 3 weeks. d, f The expression of DMP1 and DSPP was examined on gene and protein level in DPSCs from health and health with pLko.1-ALPL shRNA (d), or DPSCs from HPP and HPP with pLenti-ALPL (f) after cultured in odontoblastic medium for 7 days (n = 3). The data were presented as the mean ± s.d. of triplicate samples and analyzed by Student’s t test. *p < 0.05, **p < 0.01, ***p < 0.001