Fig. 8

Schematic diagram depicts how ALPL deficiency impairs DPSC differentiation into odontoblasts and a therapeutic method. In Healthy DPSC, ALPL is essential for regulating canonical Wnt signal pathway activation, which increases the expression of odontoblast-associated gene, DSPP and DMP1, and subsequently promotes odontoblastic differentiation of DPSC. In HPP DPSC, ALPL deficiency caused by ALPL mutation, disrupted intracellular GSK3β to β-catenin signaling cascade, then impaired odontoblastic differentiation of DPSC. While LiCl treatment can rescue the impairment of odontoblastic differentiation of HPP DPSC through reactivating intracellular GSK3β to β-catenin signaling transduction