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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Improvement of ECM-based bioroot regeneration via N-acetylcysteine-induced antioxidative effects

Fig. 1

Characterization of DFCs, establishment of H2O2-induced oxidative stress model, and determination of particular NAC treatment dose. a Characterization of DFCs. Primary DFCs were adherent to plastic and showed colony-forming abilities. TEM evaluations showed homogeneous electron-dense granules without membranous structures (indicated by yellow arrow). After being cultured in osteogenesis and adipogenesis-inducing media for 14 days and in neuronal-inducing medium for 2 h, the cultured DFCs showed mineralized nodules, lipid clusters, and positive βIII-tubulin expression, respectively. b Effect of different concentrations of H2O2 on the viability of hDFCs. c Effect of different concentrations of NAC on the viability of hDFCs. d Effect of NAC on the viability of hDFCs under oxidative stress conditions induced by 150 μm H2O2. e Effect of NAC on the proliferation of hDFCs for 1 week. f Assessment of ROS levels using fluorescence microscopy and statistical analysis of DCFH fluorescence from three experiments. Asterisks indicate statistically significant differences compared with the control group. P < 0.05, P < 0.01, and P < 0.001

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