Fig. 1
From: Generation of UCiPSC-derived neurospheres for cell therapy and its application
Characterization of iPSCs lines obtained upon reprogramming of urinary cells from normal donor. a Morphology from urinary cells to iPSCs at different time points. b Immunofluorescent labeling for pluripotency markers NANOG. c qRT-PCR assay for expression of endogenous human pluripotency genes in this iPSCs lines, with UCs as negative control and H1 as positive control (n = 3). d G-band analysis of the iPSCs showed a normal karyotype. e Teratoma formation of iPSCs lines. iPSCs injection into NOD-SCID immunodeficient mice resulted in teratoma formation (Left, n = 6). HE-staining showed that the capacity for iPSCs differentiation into three germ layers was certified by teratoma formation in vivo (right): mesoderm (cartilage, circle), endoderm (gut-like epithelium, arrow), and ectoderm (neural rosette, asterisk). **indicates P < 0.01. Scale bar: 100 μm (a); 50 μm (b, e)