Fig. 2
From: Generation of UCiPSC-derived neurospheres for cell therapy and its application
Generation of induced neural stem cells (iNSCs) from hiPSCs via single BMP inhibition. a, b Protocol timeline and representative images to describe the different stages of generating Neurospheres/iNSCs from hiPSCs. c Immunofluorescence staining with NSC markers PAX6 and Nestin. d G-band analysis of the iNSCs shows normal karyotype. e Bar graphs of qRT-PCR showing mRNA-expression profile of iNSCs respect to iPSCs (n = 3). f Bar graphs of qRT-PCR showing mRNA-expression profile of iNSCs with respect to neuron (n = 3). g Bar graphs of qRT-PCR showing mRNA-expression profile of iNSCs with respect to EB (n = 3). **indicates P < 0.01, ***indicates P < 0.001. Scale bar: 100 μm (b); 50 μm (c)