Fig. 3

Cell proliferation potential and senescence assay. a Fluorescence imaging for BrdU and DAPI labeling of three groups of cells. b The quantitative analysis of BrdU-positive percentage of three groups of cells (n = 4 per group). c Cell growth curve (n = 3 per group). d CCK8 detection of cell proliferation at different time (control and ONFH-BMSC group, n = 6, iPSC-MSC group n = 8, control vs iPSC-MSC, p > 0.05). e β-galactosidase staining for senescence assay. f The quantitative analysis of β-galactosidase staining (n = 5). All data are shown as mean ± SEM. Statistical differences were calculated using one-way ANOVA with Tukey’s adjustment (**p < 0.01; ***p < 0.0001). Scale bar indicated 50 μm in all panels