Fig. 3
Compound screening for activation of atrial and ventricular reporter genes. GATA4-targeted compounds were screened during D2–D10 of spontaneous differentiation and measured for a %atrRFP+ cells, b atrRFP-MFI, c atrial/ventricular ratio, and d atrRFP/venGFP ratio. All-trans retinoic acid (ATRA), atrRFP (SMyHC3-TdTomato, atrial), and mean fluorescent intensity (MFI). Data is presented as mean ± SEM (n = independent experiments). ****P ≤ 0.0001, ***P ≤ 0.001, **P ≤ 0.01, *P ≤ 0.05 (T test vs DMSO control). n = 2 (3i-1000 1 μM), n = 3 (3i-1229 3 μM), n = 4 (3i-1103 1 μM, 3i-1103 3 μM, 3i-1103 5 μM), n = 9 (3i-1000 3 μM, 3i-1000 5 μM), n = 10 (ATRA 10 μM)