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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Transplantation of human amniotic epithelial cells promotes morphological and functional regeneration in a rat uterine scar model

Fig. 7

The expression levels of MMP-8 increased in hAECs treated with H2O2 in vitro. a The cell viability of hAECs treated with the indicated concentrations of H2O2 for 12 h by CCK-8 assay. The cell viability of hAECs increased after exposure to 25 μM H2O2 for 12 h; however, the viability of hAECs decreased significantly with the increase of H2O2 concentration (n = 3; *P < 0.05, ***P < 0.001, ****P < 0.0001 vs 0 μM H2O2). b Total MMP-8 levels in the supernatant derived from hAECs stimulated by H2O2 (25 μM) for 12 h were quantified by ELISA (n = 3; **P < 0.01). c The expressions of MMP-8 in hAECs exposed to H2O2 (25 μM) for 12 h were measured by western blot. d The relative expression level of MMP-8 normalized to β-tubulin (n = 3; **P < 0.01). Control, cultured with a normal medium; H2O2, cultured with a medium containing 25 μM H2O2

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