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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Low-dose IL-34 has no effect on osteoclastogenesis but promotes osteogenesis of hBMSCs partly via activation of the PI3K/AKT and ERK signaling pathways

Fig. 5

The effects of IL-34 overexpression hBMSC cell line on osteogenic differentiation. a–g Expression of RUNX2, COL1A1, IL-34, P-AKT, T-AKT, P-ERK, and T-ERK was determined by Western blotting on day 3 of osteogenesis. h The results of ELISA. i-k ALP staining and activity on day 3 and ARS and quantitation on day 12 of osteogenesis, respectively. l–q The mRNA expression levels of COL1A1, RUNX2, OCN, OPN, ALP, and SP7 were evaluated by qRT-PCR. All of the experiments were independently accomplished no less than three times. Data are means ± SD. *P < 0.05 vs. the control group on day 3, @P < 0.05 vs. the control group on day 12. Scale bars, 500 μm

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Stem Cell Research & Therapy

ISSN: 1757-6512