Table 1 Sequences of primers for qRT-PCR
Primers (5′–3′) | ||
|---|---|---|
Gene | Forward | Reverse |
h-COL1A1 | CAGATCACGTCATCGCACAAC | GAGGGCCAAGACGAAGACATC |
h-RUNX2 | TGGTTACTGTCATGGCGGGTA | TCTCAGATCGTTGAACCTTGCTA |
h-OCN | CACTCCTCGCCCTATTGGC | CCCTCCTGCTTGGACACAAAG |
h-SP7 | AGCCCATTAGTGCTTGTAAAGG | CCTCTGCGGGACTCAACAAC |
h-ALP | ACCACCACGAGAGTGAACCA | CGTTGTCTGAGTACCAGTCCC |
h-OPN | CTCCATTGACTCGAACGACTC | CAGGTCTGCGAAACTTCTTAGAT |
h-18S | CCAGACAAATCGCTCCACCAAC | GACTCAACACGGGAAACCTCAC |
m-β-ACTIN | TCTGCTGGAAGGTGGACAGT | CCTCTATGCCAACACAGTGC |
m-NFATc1 | CGTTGCTTCCAGAAAATAACA | TGTGGGATGTGAACTCGGAA |
m-CATHEPSIN K | CTTCCAATACGTGCAGCAGA | TCTTCAGGGCTTTCTCGTTC |
m-C-FOS | CCAGTCAAGAGCATCAGCAA | AAGTAGTGCAGCCCGGAGTA |
m-CTR | TGCTGGCTGAGTGCAGAAACC | GGCCTTCACAGCCTTCAGGTAC |
m-MMP-9 | CAAAGACCTGAAAACCTCCAA | GGTACAAGTATGCCTCTGCCA |