Fig. 3

The dedifferentiation process of peritoneal mesothelial cells was reduced by intraperitoneal stem cell treatment. Represented IHC staining photographs were human nucleoli staining, to confirm the injection of human stem cells going to peritoneal abdomen layer; cytokeratin 18 staining, to indicate mesothelial cells of the peritoneal layer; N-cadherin staining, a mesenchymal cell marker of the peritoneal layer; α-SMA staining, to assess myofibroblast of the peritoneal layer. Cell nuclei were counterstained with hematoxylin. Data were presented as mean ± SEM. Control group n = 3 and the others n = 6. ANOVA, p < 0.05, different characters represent different levels of significance. Scale bar=50 μm. Abbreviations: CtrL control, MGO methylglyoxal, BM-MSC bone marrow-derived mesenchymal stem cell, ADSC adipose-derived mesenchymal stem cell, IHC immunohistochemistry, α-SMA alpha-smooth muscle actin