Fig. 4

Dual administration of low-dose allogeneic MSCs does not significantly alter the frequency of activated CD4+ T cells or regulatory T cells in the draining lymph nodes. Draining lymph nodes (dLNs) were harvested at D+2 from corneal allograft recipient mice receiving either two injections of PBS or low-dose allogeneic MSCs (D−1 and D+1). a Flow cytometry gating strategy used to select activated CD4+ T cells (CD4+CD25+ or CD4+CD69+) or regulatory T cells (Tregs) (CD4+CD25+Foxp3+). b Proportion of CD69+ T cells expressed as a percentage of the parent (CD4+) population. c Proportion of CD25+ T cells expressed as a percentage of the parent (CD4+) population. d Proportion of CD25+Foxp3+ Tregs expressed as a percentage of the parent (CD4+) population. Error bars: mean ± SD (each individual dot represents a separate animal, n = 4–6). D’Agostino and Pearson omnibus normality test and Shapiro-Wilk normality test used to determine distribution of data. ROUT testing was used to identify outliers. Non-parametric unpaired two-tailed Student’s t tests used for data that was not normally distributed