Fig. 4

Cell cycle and apoptosis analysis. Images of injected femur bone marrow stained for GFP (greed), DAPI (blue), and Ki-67 (red) at week 1 and week 4 in non-irradiated (a, b) and irradiated mice (c, d). Scale bars = 20 μm. e Quantification of Ki-67⁺ hBMSC from non-irradiated animals (blue, week 1 n = 7, week 4 n = 4), and 2 Gy irradiated animals (red, week 1 n = 7, week 4 n = 6). Horizontal bars represent averages, each point represents an individual mouse. Image of femur bone marrow from stained for GFP (green), DAPI (blue), and TUNEL (red) at week 1 and week 4 in non-irradiated (f, g) and irradiated mice (h, i). Scale bar = 20 μm. j Quantitative analysis of TUNEL staining in hBMSC-Luc/GFP (non-irradiated animals are represented in blue (week 1 n = 7, week 4 n = 4) and 2 Gy irradiated animals are in red (week 1 n = 7, week 4 n = 6). Horizontal bars represent averages, each point represents an individual mouse. Statistical significance calculated by two-way ANOVA with Holm-Sidak’s multiple correction using a 95% confidence level, significance identified by asterisk, * p ≤ 0.05. * p ≤ 0.05, ** p ≤ 0.01