Fig. 2

Biochemical analysis of tissue and serum in a murine model of chronic kidney disease (CKD). Sham mice were placed on a normal phosphate (0.5%) diet and CKD mice were placed on high-phosphate (1.5%) diet for 12 weeks with either PBS or exosome treatment. a Identification of PKH26-labeled exosomes in the kidney tissues. Scale bar = 100 μm. b Masson-trichrome staining of kidneys. Scale bar = 100 μm. c Quantitative analysis of the interstitial fibrosis area. Serum concentrations of blood urea nitrogen (BUN, d), creatinine (Cre, e), calcium (Ca, f), phosphorus (Pi, g), FGF23 (h), IL-6 (i), and IL-10 (j) were measured by ELISA. n = 6 per group. Data are presented as the mean ± SD. *p < 0.05, **p < 0.01, compared with the sham group. ^#p < 0.05 compared with the PBS group