Fig. 2
From: TRPC7 regulates the electrophysiological functions of embryonic stem cell-derived cardiomyocytes
Knockdown of TRPC7 decreased while overexpression of TRPC7 increased the frequency of calcium transients (CaTs) in mESC-CMs. Representative CaTs of mESC-CMs recorded from a knockdown control (shRNA-luciferase) and b TRPC7 knockdown (shRNA-458) groups. Bar charts showing c the frequency, d the normalized amplitude, e the basal intensity, f the time-to-peak, g the decay constant, h the Vmax-upstroke, and I the Vmax-decay of CaTs from a and b. Knockdown of TRPC7 decreased the frequency, Vmax-upstroke and Vmax-decay, increased the time-to-peak and the decay constant and did not change the amplitude and the basal intensity of CaTs. Representative CaTs of mESC-CMs recorded from J overexpression control (BFP only) and k TRPC7 overexpression groups. Bar charts showing l the frequency, m the normalized amplitude, n the basal intensity, o the time-to-peak, p the decay constant, q the Vmax-upstroke, and r the Vmax-decay of CaTs from j and k. Overexpression of TRPC7 increased the frequency, Vmax-upstroke, and Vmax-decay; decreased the time-to-peak and the decay constant; and did not change the amplitude and the basal intensity of CaTs. Data were presented as mean ± SEM (n = 17–19 cells; cells were from 3 independent batches of differentiation). *P < 0.05, **P < 0.01 vs corresponding controls