Fig. 4

Knockdown of TRPC7 decreased while overexpression of TRPC7 increased the frequency of action potentials (APs) in mESC-CMs. Representative APs of mESC-CMs recorded from a knockdown control (shRNA-luciferase) and b TRPC7 knockdown (shRNA-458) groups. Bar charts showing c the frequency, d the diastolic depolarization rate (DDR), e the amplitude, f the 50% action potential duration (APD₅₀), and g the maximum diastolic potential (MDP) of APs from a and b. Knockdown of TRPC7 decreased the frequency and DDR without affecting the amplitude, APD₅₀ and MDP of APs. Representative APs of mESC-CMs recorded from h overexpression control (BFP only) and i TRPC7 overexpression groups. Bar charts showing j the frequency, k the DDR, l the amplitude, m APD₅₀, and n the MDP of APs from h and i. Overexpression of TRPC7 increased the frequency and DDR without affecting the amplitude, APD₅₀ and MDP of APs. Data were presented as mean ± SEM (n = 11–14 cells; cells were from 5 independent batches of differentiation). *P < 0.05, **P < 0.01 vs corresponding controls