Fig. 4
M(IFNG/LPS) macrophage impair endogenous fatty acid synthesis in ASC. a Simplified overview of the glycolytic and fatty acid synthesis pathways with enzymes analyzed on protein level (marked in green). b, c mRNA levels of glycolysis - (b) and endogenous FA-synthesis regulating (c) enzymes analyzed in day 14 CM-differentiated adipocytes. Data were normalized to the 18S rRNA reference gene and shown as mean –ΔCt, n=3. d, e Representative immunoblots of enzymes regulating glycolysis (d) or lipid metabolism (e) of day 14 differentiated adipocytes. f Mitochondrial activity of day 14 differentiated adipocytes assessed by resazurin-based fluorescence assay (PrestoBlueTM), n=4. g Cell counts of day 14 differentiated adipocytes shown as relative numbers compared to Mo-CM-treated cells, n=4. Asterisks indicate p values <0.05 (*), <0.01 (**), <0.001 (***), and <0.0001 (****). Statistical significance was determined by using one-way ANOVA and Dunnett’s test for multiple comparison. Data are shown as mean ± SEM