Table 2 Mouse primer sequences using quantitative real time polymerase chain reaction
Genes | Primer sequences | Tm | |
|---|---|---|---|
C/ebpα | Forward | 5′-CGCAAGAGCCGAGATAAAGC-3′ | 60 |
Reverse | 5′-CGGTCATTGTCACTGGTCAACT-3′ | ||
Leptin | Forward | 5′-GACACCAAAACCCTCATCAAGAC-3′ | 60 |
Reverse | 5′-CGTGTGTGAAATGTCATTGATCCT-3′ | ||
Adiponectin | Forward | 5′-GGAACTTGTGCAGGTTGGAT-3′ | 55 |
Reverse | 5′-CCTTCAGCTCCTGTCATTCC-3′ | ||
Fabp4 | Forward | 5′-TCGATGAAATCACCGCAGAC-3′ | 50 |
Reverse | 5′-TGTGGTCGACTTTCCATCCC-3′ | ||
Hmgcr | Forward | 5′-CACCTCTCCGTGGGTTAAAA-3′ | 60 |
Reverse | 5′-GAAGAAGTAGGCCCCCAATC-3′ | ||
Icam-1 | Forward | 5′-AACAGAATGGTAGACAGCAT-3′ | 60 |
Reverse | 5′-TCCACCGAGTCCTCTTAG-3′ | ||
Il-1β | Forward | 5′-GCCACCTTTTGACAGTGATGAG-3′ | 55 |
Reverse | 5′-CCTGAAGCTCTTGTTGATGTGC-3′ | ||
Il-6 | Forward | 5′-TCTATACCACTTCACAAGTCGGA-3′ | 60 |
Reverse | 5′-GAAT TGCCATTGCACAACTCTTT-3′ | ||
Tnf-α | Forward | 5′-GTCTACTGAACTTCGGGGTGA-3′ | 60 |
Reverse | 5′-CTCCTCCACTTGGTGGTTTG-3′ | ||
Tgf-β2 | Forward | 5′-TCGACATGGATCAGTTTATGCGCA-3′ | 60 |
Reverse | 5′-CCCTGGTACTGTTGTAGATGGA-3′ |